Prostate Cancer Field Effect. Homo sapiens

In this study, comparison of gene expression profiles in benign epithelia from men with prostate cancer to those of men without prostate cancer reveal differences in several genes associated with prostate cancer. Overall design: Custom Agilent 44K whole human genome expression oligonucleotide microarrays were used to profile benign epithelium from prostate needle biopsies from 15 men with high grade(Gleason 8-10) prostate cancer and 14 age- and BMI-matched controls. All samples were laser-capture microdissected and total RNA isolated and amplified prior to hybridization against a common reference pool of prostate tumor cell lines本研究通过对比前列腺癌男性患者与非前列腺癌男性的良性上皮基因表达谱，发现了多个与前列腺癌相关的基因表达差异。 实验设计：采用定制安捷伦（Agilent）44K全人类基因组表达寡核苷酸微阵列，对15例高级别（Gleason评分8-10）前列腺癌男性患者的前列腺穿刺活检样本中的良性上皮组织，以及14例年龄与身体质量指数（BMI）匹配的对照个体的对应良性上皮组织进行表达谱分析。所有样本均经激光捕获显微切割（laser-capture microdissection）处理，在与通用前列腺肿瘤细胞系参考池进行杂交前，先完成总RNA的提取与扩增。