Additional file 1 of Functional transcriptome analyses of Drosophila suzukii midgut reveal mating-dependent reproductive plasticity in females

Additional file 1: Supplementary Table S1. Alignment statistics of the virginand mated D. suzukii female midgut RNA-Seq analysis. Supplementary Table S2. All genes description and FPKM value in D.suzukii virgin and mated female midguttranscriptomes. Supplementary Table S3. Differentially expressed genes in pairwise comparison in D. suzukii virgin and mated female midguttranscriptomes. Supplementary Table S4. GO classification of the differentially expressed genes in pairwise comparisonin D. suzukii virgin and mated femalemidguttranscriptomes. Three main categories, namely biological process (BP), cellularcomponent (CC), and molecular function (MF) were assigned to DEGs. Supplementary Table S5. KEGG pathwayenrichment analysis for differentially expressed genes in pairwise comparisonin D. suzukii virgin and mated femalemidguttranscriptomes. Supplementary Table S6. Primersused in our study. Supplementary FigureS1. Post-mating change in midgut length 1 and 3 days after mating in D.suzukii. Midgut length quantifications (A) and (D), representative imagesof virgin and mated female midgut phenotypes (B) and (E), changes in midgutrevealed by DAPI staining (C) and (F). The scale label is 500 um in picture Band E, and 20 um in picture C and F. SupplementaryFigure S2. Mating increases cell proliferation in female D. suzukii midgut.Error bars indicate the SEM of three independent biological replicates andasterisks (**) indicate the statistically significant differences (P < 0.01)between virgin and mated female midgut based on Student’ s t-test.SupplementaryFigure S3. Evaluation of sequence quality for the D. suzukii virgin and mated female midguttranscriptomes. SupplementaryFigure S4. Distribution of protein coding genes lengths in D.suzukii virgin and mated female midguttranscriptomes. The sizes of all protein coding genes were calculated. SupplementaryFigure S5. Mating increases neutrallipidcontent revealed by Bodipy staining in the whole virgin and mated female midgutof D. suzukii. The quantification is showed in the right. The scale bar is 500 um.

附加文件1：补充表S1。斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠RNA测序分析的比对统计数据。
补充表S2：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组中所有基因的描述及FPKM值。
补充表S3：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组两两比较得到的差异表达基因（DEGs）。
补充表S4：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组两两比较所得差异表达基因（DEGs）的GO功能分类。差异表达基因（DEGs）被划分为生物过程（BP）、细胞组分（CC）与分子功能（MF）三大类别。
补充表S5：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组差异表达基因（DEGs）的京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析结果。
补充表S6：本研究中使用的引物序列。
补充图S1：斑翅果蝇（D. suzukii）交配后1天与3天的中肠长度变化。包含中肠长度量化结果(A)与(D)、处女雌蝇与交配雌蝇中肠表型的代表性图像(B)与(E)、经4',6-二脒基-2-苯基吲哚（DAPI）染色后显示的中肠变化(C)与(F)。图B与图E的标尺为500 μm，图C与图F的标尺为20 μm。
补充图S2：交配可促进斑翅果蝇（D. suzukii）雌蝇中肠的细胞增殖。误差棒代表三次独立生物学重复的标准误（Standard Error of the Mean，SEM），星号(**)表示基于学生t检验（Student’s t-test）得到的处女雌蝇与交配雌蝇中肠之间存在极显著统计学差异（P < 0.01）。
补充图S3：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组的序列质量评估结果。
补充图S4：斑翅果蝇（D. suzukii）处女雌蝇与交配雌蝇中肠转录组中蛋白编码基因的长度分布。本研究统计了所有蛋白编码基因的序列长度。
补充图S5：通过Bodipy染色显示交配可提升斑翅果蝇（D. suzukii）整只处女雌蝇与交配雌蝇中肠内的中性脂质含量。右侧为量化结果，标尺为500 μm。