Genome wide microarray based expression profiles associated with BmNPV resistantance and susceptibility in Indian silkworm races of Bombyx mori

The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in the midgut tissues of BmNPV infected resistant (Sarupat) and susceptible (CSR-2) Indian silkworm races. In the resistant race, 735 genes were upregulated and 589 genes were down regulated at 12 hours of BmNPV post infection. Similarly in case of susceptible race, 2183 genes were up regulated and 2115 genes were down regulated. Among the differentially expressed genes, nine upregulated and eight down regulated genes were validated using real time qPCR analysis. In Sarupat, the significantly upregulated genes are vacuolar protein sorting associated gene, X fin like protein and Carboxy peptidase E like protein in BmNPV infected midguts, whereas the prominent down regulated genes are glutamate receptor ionotropic kainite 2-like, BTB/POZ domain and transferrin. In the case of CSR-2, the considerably upregulated genes are Peptidoglycan recognition protein S6 precursor and rapamycin while the conspicuous down regulated genes are facilitated trehalose transporter and zinc transporter ZIP1-like gene. Our results provided a vital insights of Bombyx mori in reference with its molecular mechanism in immune response against the BmNPV invasion. Organism : Bombyx mori , Agilent Custom Silkworm Gene Expression 4x44k Array (AMADID: 066047) designed by Genotypic Technology Private Limited.

本研究针对家蚕抗BmNPV的分子机制展开探究，以感染家蚕核型多角体病毒（Bombyx mori nucleopolyhedrovirus，BmNPV）的印度家蚕抗性品系（Sarupat）与感性品系（CSR-2）的中肠组织为样本，采用全基因组芯片（genome-wide microarray）进行实验。在抗性品系中，BmNPV感染后12小时，共有735个基因上调表达，589个基因下调表达。同理，在感性品系中，上调表达基因达2183个，下调表达基因达2115个。在差异表达基因中，研究团队通过实时定量聚合酶链式反应（real-time qPCR）验证了9个上调基因与8个下调基因。在Sarupat品系的BmNPV感染中肠组织内，显著上调表达的基因为囊泡蛋白分选相关基因（vacuolar protein sorting associated gene）、X fin样蛋白（X fin like protein）以及羧肽酶E样蛋白（Carboxy peptidase E like protein）；而显著下调表达的基因为离子型谷氨酸受体红藻氨酸2样蛋白（glutamate receptor ionotropic kainite 2-like）、BTB/POZ结构域蛋白（BTB/POZ domain）以及转铁蛋白（transferrin）。在CSR-2品系中，显著上调表达的基因为肽聚糖识别蛋白S6前体（Peptidoglycan recognition protein S6 precursor）与雷帕霉素（rapamycin）；而显著下调表达的基因为易化海藻糖转运蛋白（facilitated trehalose transporter）与锌转运蛋白ZIP1样基因（zinc transporter ZIP1-like gene）。本研究结果为解析家蚕（Bombyx mori）抗BmNPV侵染的免疫应答分子机制提供了重要见解。实验生物：家蚕（Bombyx mori）；实验所用的安捷伦定制家蚕基因表达4×44k芯片（Agilent Custom Silkworm Gene Expression 4x44k Array，AMADID编号：066047）由Genotypic Technology Private Limited公司设计。