Transcriptional Regulators SP110 and SP140 Modulate Inflammatory Response Genes in Mycobacterium tuberculosis-Infected Human Macrophages

Transcriptional regulatory genes SP110 and SP140 are the homologs of Sp110 and Sp140, respectively, encoded within the mouse super susceptibility to tuberculosis 1 (Sst1) locus. we investigated the gene regulatory function of SP110 and SP140 in human macrophages infected with Mycobacterium tuberculosis. The genome-wide transcriptional profile of THP-1 macrophages revealed that the induction of gene expression associated with inflammatory responses, IFN response genes, upon M. tuberculosis infection were impaired by the depletion of SP110 and/or SP140 genes. suggesting human SP110 and SP140 genes as positive regulator for genes associated with inflammatory responses in M. tuberculosis-infected macrophages.

转录调控基因（Transcriptional regulatory genes）SP110与SP140分别为Sp110和Sp140的同源基因，二者均由小鼠结核超易感1（Sst1）基因座编码。本研究探究了SP110与SP140在感染结核分枝杆菌（Mycobacterium tuberculosis）的人巨噬细胞中的基因调控功能。对THP-1巨噬细胞的全基因组转录谱分析显示，结核分枝杆菌感染诱导的炎症应答相关基因与干扰素应答基因的表达过程，会因SP110和/或SP140基因的敲低而受到削弱。上述结果提示，人源SP110与SP140基因可作为结核分枝杆菌感染巨噬细胞中炎症应答相关基因的正向调控因子。