Genome-wide analysis of day 31 hESC-cardiomyocytes treated with DMSO (control) or retinoic acid (RA) during differentiation. Homo sapiens

Human embryonic stem cell-reporter line hESC-NKX2.5(eGFP/w) were differentiated to cardiomyocytes (CMs) by utilizing the spin embryoid body method. During differentiation the cells were treated with DMSO or retinoic acid (RA) from day 4-7. At day 31, cells were sorted based on GFP prior to RNA isolation. The results of this microarray demonstrate that CMs treated with RA during differentiation exhibit atrial-like gene expression profile, while DMSO-treated cells show ventricular-like gene profile. Overall design: CMs treated with DMSO or RA during differentiation were sorted for GFP and analyzed for differential gene expression.

本数据集采用人胚胎干细胞（human embryonic stem cell）报告细胞系hESC-NKX2.5(eGFP/w)，通过悬浮拟胚体（spin embryoid body）法将其诱导分化为心肌细胞（cardiomyocytes，CMs）。分化过程中，于第4至7天分别用二甲基亚砜（dimethyl sulfoxide，DMSO）或视黄酸（retinoic acid，RA）处理细胞。在分化第31天时，基于绿色荧光蛋白（green fluorescent protein，GFP）对细胞进行分选，随后提取RNA。本次微阵列（microarray）分析结果显示：分化阶段经视黄酸处理的心肌细胞呈现心房样基因表达谱，而二甲基亚砜处理组细胞则表现出心室样基因表达特征。实验整体设计：对分化过程中经二甲基亚砜或视黄酸处理的心肌细胞分选绿色荧光蛋白阳性细胞，对其差异基因表达进行分析。