Gene expression profile at single cell level on cells from the lungs of mice infected with Mycobacterium tuberculosis

We used single-cell RNA sequencing (scRNAseq) to compare cells from the lungs of Mycobacterium tuberculosis infected mice between C57BL/6J mice and a cohort of wild-derived mouse lines MANA, MANB and MANC (Jackson Laboratories: ManA/NachJ #035354; ManB/NachJ #035355). Lung lobes were pooled from five infected mice per genotype and cells were isolated for analysis by scRNAseq.

本研究采用单细胞RNA测序（single-cell RNA sequencing，缩写为scRNAseq）技术，对比结核分枝杆菌（Mycobacterium tuberculosis）感染的C57BL/6J小鼠与一组野生来源小鼠品系MANA、MANB、MANC（杰克逊实验室：ManA/NachJ #035354；ManB/NachJ #035355）的肺部细胞。我们将每种基因型的5只感染小鼠的肺叶混合，分离细胞后开展scRNAseq分析。