Manikaran hot springs water metagenome. Water metagenome

Metagenomic profiling of a stressed niche provides insights into its taxonomic and functional potential. Natural geothermal springs represent a unique and extreme environment with adverse conditions of temperature and salinity. Here, a metagenome-based approach was selected to analyze microbiome inhabiting bubbling water samples of a geothermal spring having highest temperature across India. Analysis of community DNA showed that the phyla Proteobacteria, Thermi, Actinobacteria, Crenarchaeota and Bacteroidetes were most abundant. Functional analysis revealed high enrichment of genes for repair system and homologous recombination suggesting that the microbial communities thriving in such an extreme environment have evolved extensive DNA repair systems to withstand high temperature. Also, a de novo genotype of Emticicia oligotrophica strain MM was reconstructed and validated from metagenomic reads.

对胁迫生境开展宏基因组表征分析，可为解析其分类学特征与功能潜能提供重要研究视角。天然地热泉属于独特的极端环境，其温度与盐度条件均较为严苛。本研究针对印度境内温度最高的一处地热泉的冒泡水样，采用宏基因组学分析方法解析其中栖息的微生物组。群落DNA分析结果显示，变形菌门（Proteobacteria）、热变形菌门（Thermi）、放线菌门（Actinobacteria）、泉古菌门（Crenarchaeota）与拟杆菌门（Bacteroidetes）为该生境中的优势菌门。功能富集分析结果表明，修复系统与同源重组相关基因呈现显著富集，这表明在此类极端环境中存活的微生物群落，已演化出完善的DNA修复系统以抵御高温胁迫。此外，本研究从宏基因组测序读段中从头组装并验证了贫养埃姆蒂西亚菌（Emticicia oligotrophica）MM菌株的全新基因型。