Different mutants impact on the transcriptome response to nutrients depletion

Mutating genes either from the U1 snRNP or U2 snRNP modified the expression of a common set of genes in the stationary phase of growth. Overall design: RNA extracted from wild type cells (WT) of Saccharomyces cerevisiae and tor1 delta, nam8 delta, mud1 delta and hsh49::Q24P mutants was sequenced and their expression pattern compared in different phases of growth.

源自U1小核糖核蛋白颗粒（U1 snRNP）或U2小核糖核蛋白颗粒（U2 snRNP）的突变基因，可改变酿酒酵母（Saccharomyces cerevisiae）生长稳定期内共同基因集的表达水平。
总体实验设计：提取酿酒酵母野生型（WT）细胞以及tor1 delta、nam8 delta、mud1 delta与hsh49::Q24P突变体的RNA进行测序，并比较不同生长阶段下各样本的基因表达模式。