RNA-sequencing of innate lymphoid cells that were sorted from mouse skin

Purpose: Transcriptomic analysis of skin innate lymphoid cells Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice, respectively and subjected to RNA isolation and library preparation. Results: 3,078 differentially expressed genes (fold change>2 and p-value<0.05) were detected Conclusions: This study reveals transcriptome landscape of skin innate lymphoid cells Overall design: Transcriptome profiles of skin innate lymphoid cells were generated by RNA-seq in tripricate研究目的：开展皮肤固有淋巴细胞（skin innate lymphoid cells）的转录组分析。 实验方法：分别从成年B6小鼠的表皮、真皮及皮下组织中分选固有淋巴细胞，随后进行RNA提取与文库制备。 实验结果：共检测到3078个差异表达基因（倍数变化>2且p值<0.05）。 研究结论：本研究揭示了皮肤固有淋巴细胞的转录组图谱。 整体实验设计：通过RNA-seq对皮肤固有淋巴细胞开展三次生物学重复的转录组谱分析。