A non-canonical R-loop resolving function of PIWIL4 maintains the genomic integrity and leukemic growth of AML cells

PIWIL4 enzyme is required for resolving R-loops and for growth of AML cells. We examine the impact of PIWIL4 knockdown and R-loop accumulation on gene expression in AML cell line THP-1 Overall design: Examination of differential gene expression upon PIWIL4 knockdown in AML cells

PIWIL4酶（PIWIL4）对于解离R环（R-loop）以及急性髓系白血病（Acute Myeloid Leukemia，AML）细胞的增殖不可或缺。本研究考察了PIWIL4敲低与R环积累对AML细胞系THP-1内基因表达的影响。整体实验设计：检测AML细胞中PIWIL4敲低后的差异基因表达情况。