DataSheet1_A nanohybrid synthesized by polymeric assembling Au(I)-peptide precursor for anti-wrinkle function.docx

A major sign of aging is wrinkles (dynamic lines and static lines) on the surface of the skin. In spite of Botulinum toxin’s favorable therapeutic effect today, there have been several reports of its toxicity and side effects. Therefore, the development of an effective and safe wrinkle-fighting compound is imperative. An antioxidant-wrinkle effect was demonstrated by the peptide that we developed and synthesized, termed Skin Peptide. Aiming at the intrinsic defects of the peptide such as hydrolysis and poor membrane penetration, we developed a general approach to transform the Skin Peptide targeting intracellular protein-protein interaction into a bioavailable peptide-gold spherical nano-hybrid, Skin Pcluster. As expected, the results revealed that Skin Pcluster reduced the content of acetylcholine released by neurons in vitro, and then inhibit neuromuscular signal transmission. Additionally, human experiments demonstrated a significant de-wrinkle effect. Moreover, Skin Pcluster is characterized by a reliable safety profile. Consequently, anti-wrinkle peptides and Skin Pcluster nanohybrids demonstrated innovative anti-wrinkle treatments and have significant potential applications.

皮肤表面的皱纹（动态纹与静态纹）是衰老的典型表征之一。尽管当前肉毒杆菌毒素（Botulinum Toxin）拥有良好的治疗效果，但已有多篇报道指出其存在毒性与副作用。因此，开发安全有效的抗皱化合物迫在眉睫。我们开发合成的一款命名为Skin Peptide的肽类物质，被证实具备抗氧化与抗皱功效。针对该肽类易水解、膜穿透性差等固有缺陷，我们开发了一种通用策略，将靶向细胞内蛋白质-蛋白质相互作用的Skin Peptide转化为可生物利用的肽-金球形纳米杂化物Skin Pcluster。正如预期，实验结果表明，Skin Pcluster可降低体外培养神经元释放的乙酰胆碱含量，进而抑制神经肌肉信号传导。此外，人体试验证实其具备显著的祛皱效果。更为重要的是，Skin Pcluster展现出可靠的安全性。综上，抗皱肽类与Skin Pcluster纳米杂化物为抗皱治疗提供了创新方案，具备极高的应用潜力。