Concentration of recombinant Shh in culture medium from signaling assays in transfected NIH3T3 fibroblasts.

Shown is the amount of recombinant Shh protein measured by ELISA in the culture medium from transfected NIH3T3 fibroblasts. The concentrations of recombinant Shh protein recovered in the culture media are well below the EC50 measured for each type of variant when added exogenously to NIH3T3 cells.

本数据集呈现了转染NIH3T3成纤维细胞的培养液中，经酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay，ELISA）检测得到的重组Sonic Hedgehog（Shh）蛋白含量。回收于该培养液中的重组Shh蛋白浓度，远低于每种变体外源性添加至NIH3T3细胞时所测得的半最大效应浓度（EC50）。