ADAD2 facilitates piRNA biogenesis and spermiogenesis in mice

Adenosine Deaminase Domain Containing 2 (ADAD2) is a testis-specific protein composed of a double-stranded RNA binding domain and a non-catalytic adenosine deaminase domain. A recent study showed that ADAD2 is indispensable for the male reproduction in mice. However, its detailed function remains elusive. The aims of this study are to investigate the cause of male sterility in Adad2 knockout mice and to understand the molecular function of ADAD2. RNA-seq analyses were carried out to analyze the changes in the transcriptome and piRNA population of Adad2 mutant testes. RNA-seq of testis from Adad2-knockout mouse

含腺苷脱氨酶结构域2（Adenosine Deaminase Domain Containing 2, ADAD2）是一种睾丸特异性蛋白，由双链RNA结合结构域与非催化型腺苷脱氨酶结构域组成。近期研究表明，ADAD2对小鼠的雄性生殖功能不可或缺，但其详细功能仍未明确。本研究旨在探究Adad2基因敲除小鼠雄性不育的成因，并解析ADAD2的分子功能。研究通过RNA测序（RNA-seq）分析了Adad2突变型睾丸的转录组与Piwi互作RNA（piRNA）群体的变化。本次研究还对Adad2基因敲除小鼠的睾丸进行了RNA-seq分析。