Function and hormonal regulation of GATA3 in human first trimester placentation. Homo sapiens

The transcription factor GATA3 is down regulated by estrogen and plays a role in trophoblast cell function and placentation. Overall design: To identify downstream genes involved in GATA3 regulation of trophoblast function, we performed RNA sequencing of GATA3 knockdown HTR8/SVneo cells. First, GATA3 expression in HTR8/SVneo cells were knocked down by siRNA transfection and the reduction in GATA3 expression confirmed. Then, gene expression profiles of GATA3 knockdown cells were determined using RNA sequencing on the Illumina NextSeq 500.

转录因子GATA3（transcription factor GATA3）可被雌激素下调，并在滋养层细胞功能调控与胎盘形成过程中发挥作用。整体实验设计：为鉴定参与GATA3调控滋养层细胞功能的下游靶基因，我们对GATA3敲低的HTR8/SVneo细胞开展了RNA测序（RNA sequencing）实验。首先通过siRNA转染技术敲低HTR8/SVneo细胞内的GATA3表达，并验证了GATA3表达的下调效果；随后，使用Illumina NextSeq 500测序平台对GATA3敲低细胞的基因表达谱进行检测分析。