ChIP-Seq to identify the enrichement of histone acetylation on the promoter of FASN and ACLY in tumors with BCAA dietary interventions.

In order to provide more evidence to prove that BCAAs catabolism mediates the expressions of FASN and ACLY by altering histone acetylation in melanoma, ChIP-seq of xenografted A2058 tumors implanted in mice receiving BCAA dietary interventions (normal or high BCAA diet) was employed to idenfity the enrichment of histone acetylation on the promoter of FASN and ACLY. Chromatin immunoprecipitation sequencing(ChIP-seq) for H3ac in A2058 tumors

为提供更多证据以证实支链氨基酸（Branched-Chain Amino Acids，BCAAs）的分解代谢通过调控黑色素瘤中的组蛋白乙酰化水平，介导脂肪酸合酶（Fatty Acid Synthase，FASN）与ATP柠檬酸裂解酶（ATP Citrate Lyase，ACLY）的表达。本研究对接受支链氨基酸膳食干预（正常膳食或高支链氨基酸膳食）的小鼠所移植的A2058细胞异种移植瘤开展染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq），以鉴定组蛋白乙酰化在FASN与ACLY启动子区域的富集情况。针对A2058肿瘤组织中组蛋白H3乙酰化（H3ac）的染色质免疫共沉淀测序（ChIP-seq）实验。