Response of potato sRNAome to PVYNTN infection [smallRNA-Seq]. Solanum tuberosum

We aimed at unraveling the role of small RNAs in a complex immune signaling network controlling defense responses that render the plant tolerant to viral infection. We have identified and quantified miRNAs, phasiRNA and virus-derived small RNAs in both, the PVYNTN-tolerant cv. Désirée and its susceptible transgenic counterpart impaired in accumulation of salicylic acid (NahG-Désirée). We have constructed a small RNA regulatory network using in silico prediction results as well as degradome sequencing to link our data to the physiological response (target transcript abundances and hormone levels). Overall design: small RNA-seq data of PVYNTN-infected (PVY1, PVY2, PVY3) and mock (mock1, mock2, mock3) inoculated potato plants of cv. Désirée and NahG-Désirée. Three plants per treatment were used.

本研究旨在解析小型RNA（small RNA）在调控植物防卫反应、赋予其抗病毒侵染耐受性的复杂免疫信号网络中的作用。本研究在马铃薯Y病毒NTN株系（PVYNTN）耐性马铃薯品种‘德西蕾’（cv. Désirée）及其水杨酸积累受损的易感转基因同源株系NahG-Désirée中，完成了微RNA（miRNAs）、相位小干扰RNA（phasiRNA）与病毒源性小型RNA的鉴定与定量分析。本研究结合计算机预测（in silico）结果与降解组测序（degradome sequencing）数据，构建了小型RNA调控网络，以将实验数据与植物生理响应（靶标转录本丰度及激素水平）进行关联。
实验设计概述：对‘德西蕾’与NahG-Désirée两个马铃薯品种的PVYNTN侵染组（PVY1、PVY2、PVY3）及模拟接种组（mock1、mock2、mock3）开展小型RNA测序（small RNA-seq），每个处理组设置3株供试植株。