Raw Plaque-2.0 infection scores of the PCL screen for RV, imaged and analysed at EPFL.
收藏资源简介:
Barcode indicates the screening plate sequence number defined by EPFL and Well Position gives the well. Plaque2.0-based infection scores are numberOfNuclei reporting the number of nuclei based on Hoechst stai¬ning, numberOfInfectedNuclei refers to the number of GFP reporter-based number of infected nu¬clei, infectionIndex is the ratio of numberOfInfectedNuclei to numberOfNuclei, the GFP reporter-based number of plaques is given by numberOfPlaques and totalVirusIntensity indicates total GFP reporter signal intensity.
条形码(Barcode)代表由瑞士联邦理工学院洛桑分校(EPFL)定义的筛选板序列号,孔位(Well Position)用于标识对应的实验孔。基于Plaque2.0的感染评分包含以下指标:细胞核总数(numberOfNuclei)指通过Hoechst染色(Hoechst staining)计数得到的细胞核总数量;感染细胞核总数(numberOfInfectedNuclei)指基于绿色荧光蛋白(GFP,Green Fluorescent Protein)报告系统计数得到的感染细胞核总数量;感染指数(infectionIndex)为感染细胞核总数与细胞核总数的比值;噬菌斑总数(numberOfPlaques)指基于GFP报告系统的噬菌斑总数量;病毒总信号强度(totalVirusIntensity)代表GFP报告信号的总强度。



