Transcriptome_of_the_olfactory_system_of_newborn_mice. Transcriptome_of_the_olfactory_system_of_newborn_mice

High-throughput RNA sequencing of the olfactory system of newborn mice. With this study we aim to assess the expression profile of the main olfactory epithelium (MOE) and the vomeronasal organ (VNO) at birth in C57BL/6J mice. Tissue was collected from P0.5 pups and three to four individuals were pooled for each sample. Three biological replicates were obtained for each organ. RNA was extracted using the Qiagen RNeasy kit using standard protocols. All six RNA samples were multiplexed together and sequenced in two lanes on the Illumina HiSeq platform. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/本研究针对新生小鼠的嗅觉系统开展高通量RNA测序，旨在评估C57BL/6J小鼠出生时的主要嗅觉上皮（main olfactory epithelium, MOE）与犁鼻器（vomeronasal organ, VNO）的表达谱。实验样本取自出生后0.5天（P0.5）的幼鼠，每个样本混合3至4只个体的组织，每个器官设置3次生物学重复。采用Qiagen RNeasy试剂盒及标准实验流程提取RNA，将全部6个RNA样本进行多重混样后，在Illumina HiSeq平台的两个测序泳道中完成上机测序。本数据集属于预发布数据。有关惠康桑格研究所（Wellcome Trust Sanger Institute）共享的预发布数据的规范使用说明（包括出版暂缓相关细则），请访问http://www.sanger.ac.uk/datasharing/ 查阅。