Transcriptomic analysis of IAA treated 3 DAG WT and er erl2 seedlings.

Proper control of Auxin localization and accumulation are essential for leaf primordia initiation. Additionally, previous work has shown that the ERECTA family of genes also control leaf initiation. In this study we seek to understand the interaction between Auxin and the er erl2 double mutant in 3 days after germination (DAG) old seedlings. WT and mutant seedlings were either treated with IAA or mock treatments for 30 minutes. RNA was isolated and poly-A enriched strand specific libraries were constructed and paired end sequencing was carried out. The mutant showed mild, yet consistent enrichment of chloroplast and carbohydrate metabolism gene ontology terms in our mutant compared to WT. However, at 30 minutes post IAA treatment, the mutant and WT did not have significantly different responses to auxin. This indicates that the ERECTA family genes do not play a significant role in the immediate and direct response to auxin. 2x2 Factorial analysis of WT and er erl2 mutants in Arabidopsis thaliana under Mock or IAA treatment

生长素（Auxin）的定位与积累的精准调控，对于叶原基起始发育至关重要。此外，已有研究表明ERECTA基因家族同样调控叶片起始过程。本研究旨在探究萌发后3天（3 days after germination, DAG）的幼苗中，生长素与er erl2双突变体之间的互作关系。我们将野生型（Wild Type, WT）与突变体幼苗分别用吲哚乙酸（Indole-3-acetic acid, IAA）或空白对照处理30分钟。提取总RNA后，构建聚腺苷酸（poly-A）富集的链特异性文库，并进行双端测序。与野生型相比，该突变体在叶绿体与碳水化合物代谢相关的基因本体（Gene Ontology, GO）条目上呈现出微弱但一致的富集现象。然而，在IAA处理30分钟后，突变体与野生型对生长素的响应并无显著差异。这表明ERECTA基因家族在生长素的即时直接响应过程中并未发挥显著作用。本实验针对拟南芥（Arabidopsis thaliana）的野生型与er erl2突变体，在空白对照与IAA处理条件下开展了2×2析因分析。