Additional file 4 of Comparison of two ASC-derived therapeutics in an in vitro OA model: secretome versus extracellular vesicles

Additional file 4: Supplementary Table 1. ASC features. Details of culture conditions (CTRL, Osteoinduction and Adipoinduction) and performed assays are reported. Supplementary Table 2. Functional enriched processes identified with STRING through OA-related keywords (Chondro-, Metabol-,Catabol-, Inflamm- and Matrix). GO: Gene ontology identifier. Supplementary Table 3.Quantification of OA-related factors in ASC-CM (n = 3) expressed as mean ± SD. Details of the biological processes in which each factors is involved, according to Supplementary Table 2, are also provided. Supplementary Table 4. Principal Component Analysis (PCA) details by XLSTAT software. Number of factors analyzed for each OA-related keyword are reported in brackets. Supplementary Table 5. nLC-MS/MS quantification of OA-related factors in ASC-CM and ASC-EV (n = 3). Factors significantly different between CM and EV samples are in bold. Proteins more abundant in EV that in CM are highlighted in gray.附加文件4：补充表1。脂肪源性干细胞（Adipose-derived Stem/Stromal Cells, ASC）特征信息，其中列明了培养条件（对照组、成骨诱导组与成脂诱导组）及所开展的检测实验的详细信息。补充表2。通过STRING数据库，以骨关节炎（Osteoarthritis, OA）相关关键词（软骨相关、代谢相关、分解代谢相关、炎症相关及细胞外基质相关）检索得到的功能富集过程，注：GO为基因本体（Gene Ontology）标识符。补充表3。脂肪源性干细胞条件培养基（ASC-Conditioned Medium, ASC-CM）中骨关节炎相关因子的定量分析结果（样本量n=3），以平均值±标准差形式呈现，同时提供了各因子所参与的生物学过程详情，详见补充表2。补充表4。使用XLSTAT软件完成的主成分分析（Principal Component Analysis, PCA）详情，各骨关节炎相关关键词所分析的因子数量已标注于方括号内。补充表5。通过纳米液相色谱-串联质谱（nLC-MS/MS）对脂肪源性干细胞条件培养基（ASC-CM）与脂肪源性干细胞细胞外囊泡（ASC-Extracellular Vesicles, ASC-EV）中骨关节炎相关因子的定量分析结果（样本量n=3），培养基与细胞外囊泡样本间存在显著差异的因子以粗体标注，在细胞外囊泡中表达量高于培养基的蛋白以灰色高亮显示。