Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

Abstract Introduction With glutamine supplementation there is better performance in the body’s defense system. Objective The aim of this study was to analyze the effect of glutamine supplementation on ligand-induced periodontal disease in rats. Material and method 48 selected male Wistar rats were divided into 4 groups (N = 12): control group (CG) including healthy animals receiving daily saline solution via gavage; glutamine group (GG) including healthy animals receiving oral glutamine supplementation by gavage at a dose of 1.5 g / kg / day; periodontitis group (PG) including animals with induced periodontal disease on both upper sides receiving daily saline solution via gavage; experimental group (EG) including animals with induced periodontal disease on both upper sides receiving daily glutamine via gavage at a dose of 1.5 g / kg / day. On the 30th day, all animals were euthanized by excess anesthetic. Result In the various analyses, the EG (116.63 ± 22.50 mm2) compared to the CG (82.32 ± 7.48 mm2) obtained a p<0.05. The PG (143.15 ± 35.24 mm2) compared to the EG group (116.63 ± 22.50 mm2 ) presented a p<0.05. The PG (143.15 ± 35.24 mm2) compared to the CG (82.32 ± 7.48 mm2) presented a p<0.05. The GG (118.09 ± 10.4 mm2) compared to the CG (82.32 ± 7.48 mm2) presented a p>0.05. Conclusion Glutamine supplementation associated with induced periodontal disease demonstrated a smaller amount of bone loss compared to the periodontitis group.

引言：补充谷氨酰胺（glutamine）可改善机体防御系统功能。
研究目的：本研究旨在分析谷氨酰胺补充对配体诱导性牙周病（periodontal disease）大鼠的影响。
材料与方法：选取48只雄性Wistar大鼠（Wistar rats），分为4组（每组n=12）：对照组（CG）为健康大鼠，每日经灌胃（gavage）给予生理盐水；谷氨酰胺组（GG）为健康大鼠，每日经灌胃给予谷氨酰胺，剂量为1.5 g/kg/天；牙周炎组（PG）为双侧上颌诱导牙周病大鼠，每日经灌胃给予生理盐水；实验组（EG）为双侧上颌诱导牙周病大鼠，每日经灌胃给予谷氨酰胺，剂量为1.5 g/kg/天。于第30天，所有大鼠均采用过量麻醉法实施安乐死。
研究结果：多项分析显示，实验组（EG，116.63±22.50 mm²）与对照组（CG，82.32±7.48 mm²）相比，差异具有统计学意义（p<0.05）；牙周炎组（PG，143.15±35.24 mm²）与实验组相比，差异具有统计学意义（p<0.05）；牙周炎组与对照组相比，差异具有统计学意义（p<0.05）；谷氨酰胺组（GG，118.09±10.4 mm²）与对照组相比，差异无统计学意义（p>0.05）。
研究结论：相较于牙周炎组，接受谷氨酰胺补充联合诱导性牙周病造模的大鼠骨丢失量更低。