Transcriptome-wide identification of ADAR1 p150-specific RNA editing sites in macrophage cell line. Mus musculus

ADARs are RNA editing enzymes that catalyze the deamination of adenosine to inosine in double-stranded RNAs. In mammals, there are two isoforms of ADAR1 including a p110 isoform, which is constitutively and ubiquitously expressed, and a p150 isoform regulated by an IFN-inducible promoter. The mutation in ADAR1 gene causes Aicardi-Goutieres syndrome (AGS), a severe autoimmune disease in human. Furthermore, the significant decrease in RNA-editing activity was found in the p150 isoform mutant associated with AGS. In this study, we will perform transcriptome-wide analysis and identify the targets of ADAR1p150 isoform.

ADARs (Adenosine Deaminases Acting on RNA) 是一类RNA编辑酶，可催化双链RNA中的腺苷脱氨生成肌苷。在哺乳动物中，ADAR1存在两种同工型：p110同工型为组成型普遍表达，p150同工型则受干扰素(Interferon, IFN)诱导型启动子调控。ADAR1基因突变可引发艾卡迪-古铁雷斯综合征（Aicardi-Goutieres syndrome, AGS），这是一种人类重症自身免疫性疾病。此外，与AGS相关的p150同工型突变体的RNA编辑活性显著降低。本研究将开展全转录组分析，鉴定ADAR1p150同工型的作用靶点。