Ionizing radiation induced hair and skin damage. Ionizing radiation induced hair and skin damage

Skin samples from back region and footpads were analyzed in 8-10 week old mice. For back skin, the hair was plucked in order to induce active growing phase(anagen). After 9 days, the mice were irradiated for 5Gy IR. Krt17 knock out, p53 knock out, and wild type mice were compared. Samples were taken at time 0 (control), 6h, and 24h post-irradiation. For footpad skin, the mice were irradiated for 40 Gy IR. Krt17 knock out and wild type mice were compared. Samples were taken at time 0 (control), 3 d, 6 d, 9 d post-irradiation. Overall design: Expression profiling by high-throughput sequencing

本研究针对8-10周龄小鼠的背部区域及脚垫皮肤样本展开分析。针对背部皮肤，通过拔毛诱导其进入活跃生长阶段（anagen）；9天后，对小鼠施以5Gy剂量的电离辐射（Ionizing Radiation, IR）。本研究对比了角蛋白17敲除（Krt17 knock out）、p53敲除及野生型小鼠的样本，并分别于辐射后0小时（对照组）、6小时及24小时采集样本。针对脚垫皮肤，对小鼠施以40Gy剂量的电离辐射（IR）；本研究对比了角蛋白17敲除及野生型小鼠的样本，并分别于辐射后0天（对照组）、3天、6天及9天采集样本。整体实验设计：通过高通量测序开展表达谱分析。