TTFV Microbiota

TTFV intervention effectively alleviates high-fat diet-induced gut microbiota dysbiosis in mice. Compared to the high-fat diet group, the low-fat diet group exhibited significantly elevated community richness indices. The high-fat diet induced microbial imbalance, with the high-fat diet group showing marked separation from the normal diet group. Conversely, the gut microbiota structures of both the low-fat and low-sugar diet groups resembled those of the normal diet group. This indicates that TTFV intervention can restore gut microbiota composition. The phylum Bacillales is associated with improved lipid metabolism disorders and hepatic steatosis; as a key polysaccharide-degrading phylum, TTFV increases the abundance of the phyla Thermodesulfovibrionales and Bacteroidetes while reducing the abundance of the phylum Pseudomonadales. Although the high-fat diet elevated the abundance of the Bacillales phylum, TTFV reduced its relative proportion to the Bacteroidetes phylum. Furthermore, TTFV enriched multiple beneficial bacterial groups, including the Spirochaetales (Vibrionaceae) and Eubacteriales, while decreasing the abundance of the Erythromonadales, which is associated with lipid metabolism disorders and inflammation (typically induced by a high-fat diet).TTFV干预可有效缓解高脂饮食诱导的小鼠肠道菌群失调。与高脂饮食组相比，低脂饮食组的菌群群落丰富度指数显著升高。高脂饮食会引发微生物群落失衡，高脂饮食组与正常饮食组呈现出显著的群落分离。与之相反，低脂与低糖饮食组的肠道菌群结构均与正常饮食组相似。这表明TTFV干预可恢复小鼠肠道菌群的组成结构。芽孢杆菌目（Bacillales）与脂质代谢紊乱及肝脂肪变性的改善相关；而TTFV作为关键的多糖降解类群，可提高热脱硫弧菌目（Thermodesulfovibrionales）和拟杆菌门（Bacteroidetes）的丰度，同时降低假单胞菌目（Pseudomonadales）的丰度。尽管高脂饮食会提升芽孢杆菌目的丰度，但TTFV可降低其相对于拟杆菌门的占比。此外，TTFV可富集多种有益菌群，包括螺旋体目（Spirochaetales）下属的弧菌科（Vibrionaceae）以及真杆菌目（Eubacteriales），同时降低与脂质代谢紊乱及炎症（通常由高脂饮食诱导）相关的赤单胞菌目（Erythromonadales）的丰度。