Triticum aestivum cv. Renan. CGD

This project provides the genome assembly of Triticum aestivum cv. Renan, common name French bread wheat. The sequencing of the wheat (Triticum aestivum L.) genome has been difficult for many years due to its large size, one of the most important among the sequenced plant species. Many initiatives aiming to obtain a reference genome have been launched in recent years, starting with a chromosome-based approach and ending with a chromosome-scale assembly published in 2018. It was a huge effort and the result of a combination of short read-based whole genome sequencing with many other resources generated on flow sorted chromosomes. Here, we report on an optimized, low-cost, long read-based assembly of the genome of the French bread wheat cultivar Renan generated by combining long-reads sequencing and long-range information. Using nanopore long reads generated using the PromethION device, we provide the most contiguous and complete assembly of a bread wheat genome to date, a resource that will be valuable for the crop community and will facilitate the rapid selection of agronomically important traits.

本项目提供了普通小麦（Triticum aestivum）栽培品种雷南（Renan，俗称法国面包小麦）的基因组组装结果。多年来，小麦（Triticum aestivum L.）基因组的测序工作一直极具挑战，因其基因组体量庞大，是已测序植物物种中极具重要性的类群之一。近年来，学界已启动多项旨在获取参考基因组的研究计划，其发展路径从基于染色体的研究方法起步，最终在2018年完成了染色体级别的基因组组装并发表。该成果凝聚了大量研究心血，结合了基于短读长的全基因组测序技术，以及诸多通过流式分选染色体获得的辅助测序资源。本研究报道了一款经优化的低成本长读长基因组组装结果，针对法国面包小麦栽培品种雷南，该组装通过整合长读长测序技术与长范围测序信息完成。本研究利用PromethION测序平台产出的纳米孔长读长数据，构建了截至目前连续性与完整性最优的面包小麦基因组组装结果。该资源将为作物学界提供重要支撑，并助力农艺性状相关的快速选育工作。