Supplementary figure S7 Raw data
收藏资源简介:
Supplementary figure S7 Raw dataThe progress of sporogenic development of strains producing ParB-HT in wild type and Dsmc background determined by the percentage of hyphae showing ParB complexes. Images of hyphal cells of the wild type control and Dsmc strains (DparAB pnatparAB-HT, KP006 and DsmcDparAB pnatparAB-HT KP007, respectively) from three time points of sporogenic development. ParB-HT was stained with TMR Direct Ligand (red) and DNA was stained with Hoechst 33342 (blue) overlaid on phase contrast (grey).
补充图S7 原始数据:本数据集以呈现ParB复合物的菌丝占比为量化判定依据,表征在野生型及Δsmc(Dsmc)背景下表达ParB-HT的菌株的孢子形成发育进程。数据集包含野生型对照菌株与Δsmc菌株(分别为DparAB pnatparAB-HT,即菌株KP006;以及ΔsmcΔparAB pnatparAB-HT,即菌株KP007)在孢子形成发育三个时间点的菌丝细胞图像。其中ParB-HT经TMR Direct Ligand(TMR直接配体)染色呈红色,DNA经Hoechst 33342(赫斯特33342)染色呈蓝色,图像叠加相差成像(灰色)通道。




