Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long-term field experiments

Soil nematode communities and food web indices can inform about the complexity, nutrient flows and decomposition pathways of soil food webs, reflecting soil quality. Relative abundance of nematode feeding and life-history groups are used for calculating food web indices, i.e. maturity index (MI), enrichment index (EI), structure index (SI) and channel index (CI). Molecular methods to study nematode communities potentially offer advantages compared to traditional methods in terms of resolution, throughput, cost and time. In spite of such advantages, molecular data have not often been adopted so far to assess the effects of soil management on nematode communities and to calculate these food web indices. Here, we used high-throughput amplicon sequencing to investigate the effects of tillage (conventional vs reduced) and organic matter addition (low vs high) on nematode communities and food web indices in ten European long-term field experiments and we assessed the relationship between nema...

土壤线虫群落与食物网指数可反映土壤食物网的复杂性、养分流动与分解路径，进而体现土壤质量。线虫取食类群与生活史类群的相对丰度，可用于计算各类食物网指数，即成熟度指数（Maturity Index, MI）、富集指数（Enrichment Index, EI）、结构指数（Structure Index, SI）与通道指数（Channel Index, CI）。相较于传统研究方法，线虫群落的分子生物学分析手段在分辨率、通量、成本与耗时方面均具备潜在优势。尽管具备此类优势，但截至目前，分子数据尚未被广泛应用于评估土壤管理措施对线虫群落的影响，以及计算上述食物网指数。本研究依托10项欧洲长期田间定位试验，采用高通量扩增子测序（high-throughput amplicon sequencing）技术，探究了耕作方式（常规耕作与减耕）与有机质添加量（低量与高量）对线虫群落及食物网指数的影响，并分析了线虫[后续内容缺失]之间的关联。