mRNA sequencing analysis of a novel model of 'NAFLD in a Dish'. mRNA sequencing analysis of a novel model of 'NAFLD in a Dish'

The goal of this study was to determine the changes to cellular transcriptional programs following induction of intracellular lipid accumulation, with the aim of confirming the utility of this model for exploring mechanisms underlying the pathogenesis of NAFLD. Comparisons with other publically available data confirm that the alterations we observe in mRNA expression are similar to those observed in both humans and rodents. Overall design: mRNA profiles were generated for ES-derived hepatocyte-like cells following treatment with lactate (10 mM), pyruvate (1 mM) and octanoate (2 mM) to induce intracellular lipid accumulation, resulting in a NAFLD-like phenotype

本研究旨在明确细胞内脂质蓄积诱导后细胞转录程序的改变，旨在验证该模型用于探究非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease, NAFLD）发病机制的实用性。与其他公开可用数据集的比对结果证实，本研究中观测到的mRNA表达变化，与人类及啮齿类动物中的相关变化高度相似。实验整体设计：以乳酸（10 mM）、丙酮酸（1 mM）及辛酸盐（2 mM）处理胚胎干细胞（Embryonic Stem, ES）来源的肝样细胞，诱导其发生细胞内脂质蓄积，进而形成类非酒精性脂肪性肝病表型，并以此生成对应的mRNA表达谱。