CD8+ T lymphocytes in hypopigmented mycosis fungoides: malignant cells or reactive clone?. CD8+ T lymphocytes in hypopigmented mycosis fungoides: malignant cells or reactive clone?

We used targeted single cell RNA sequencing using a gene set composed of the BD Rhapsody Human Immune Response Panel, TCR panel, and a custom panel of 61 malignancy-associated genes and T/B cell receptor sequencing to characterize single cell populations in hypopigmented mycosis fungoides (HMF). Using a reference mapping model built on publically deposited classic mycosis fungoides single cell RNA sequencing datasets, we identified a predicted malignant cell population independent from the clonal T cell population in HMF. Overall design: Sequencing of skin cells from hypopigmented patches from one patient collected as three punch biopsies. Raw data not provided due to patient privacy concerns.

本研究采用靶向单细胞RNA测序（targeted single cell RNA sequencing）技术，以BD Rhapsody人类免疫应答组合、T细胞受体（TCR）组合以及包含61个恶性相关基因的自定义基因组合作为基因集，并结合T/B细胞受体测序，对色素减退性蕈样肉芽肿（hypopigmented mycosis fungoides, HMF）中的单细胞群体进行表征。本研究基于公开存储的经典蕈样肉芽肿单细胞RNA测序数据集构建参考映射模型，在此模型中鉴定出独立于HMF内克隆性T细胞群体的预测性恶性细胞群体。整体实验设计：从1名患者的色素减退斑皮损处获取皮肤细胞，通过3份穿刺活检标本完成测序。由于患者隐私保护相关要求，未提供原始测序数据。