gut metagenome

For intestinal histology, segments of the jejunum and cecum were gently rinsed three times with ice-cold saline, sheared into 1 cm segments, and then fixed in a 4 paraformaldehyde solution. Additionally, jejunal and cecal samples were collected in sterile sampling tubes and stored at -80 for subsequent analysis针对肠道组织学检测，取空肠(jejunum)与盲肠(cecum)组织段，以冰生理盐水轻柔冲洗三次，剪切为1厘米长度的组织段后，置于4%多聚甲醛(paraformaldehyde)溶液中固定。此外，将空肠与盲肠样本收集至无菌采样管内，于-80℃条件下保存，用于后续相关分析。