x-ray structure of NeoB from streptomyces fradiae in complex with PLP and neomycin (as the external aldimine) at pH 9

x-ray structure of NeoB from streptomyces fradiae in complex with PLP and neomycin (as the external aldimine) at pH 9 Descriptor: (1R,2R,3S,4R,6S)-4,6-diamino-2-[(3-O-{2-amino-2,6-dideoxy-6-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-alpha-D-glucopyranosyl}-beta-D-ribofuranosyl)oxy]-3-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside, 1,2-ETHANEDIOL, CHLORIDE ION, ... Authors: Thoden, J.B, Dow, G.T, Holden, H.M. Deposit date: 2018-02-03 Release date: 2018-02-21 Last modified: 2023-10-04 Method: X-RAY DIFFRACTION (1.65 Å) Cite: The three-dimensional structure of NeoB: An aminotransferase involved in the biosynthesis of neomycin. Protein Sci., 27, 2018

弗氏链霉菌（Streptomyces fradiae）来源的NeoB与磷酸吡哆醛（PLP）及新霉素形成外醛亚胺复合物，在pH 9条件下的X射线晶体结构。配体描述：(1R,2R,3S,4R,6S)-4,6-二氨基-2-[(3-O-{2-氨基-2,6-二脱氧-6-[({3-羟基-2-甲基-5-[(膦酰氧基)甲基]吡啶-4-基}甲基)氨基]-α-D-吡喃葡萄糖基}-β-D-呋喃核糖基)氧基]-3-羟基环己基 2,6-二氨基-2,6-二脱氧-α-D-吡喃葡萄糖苷、1,2-乙二醇、氯离子…… 作者：Thoden, J.B.、Dow, G.T.、Holden, H.M.。提交日期：2018-02-03；发布日期：2018-02-21；最后修改日期：2023-10-04。实验方法：X射线衍射（分辨率1.65埃）。引用文献：《NeoB的三维结构：一种参与新霉素生物合成的氨基转移酶》，《Protein Science》，第27卷，2018年