Liquid Chromatography-Mass Spectrometry (LC-MS) data of a Multi-Epitope Peptibody with bFGF/VEGFA

Peptibody was treated with six proteolytic enzymes (trypsin, chymotrypsin, Asp-N, Glu-C, Lys-C and Lys-N) and digested into amenable peptide fragments for LC-MS analysis. The mass spectrometer used was Q Exactive (Thermo Scientific). For sequencing and peptide-mapping, the data were processed by BiopharmaLynx 1.3 sofware. For molecular weight (MW), the data were calculated through deconvolution by ProMass for XcaliburTM software.将肽体（Peptibody）经六种蛋白水解酶（胰蛋白酶、胰凝乳蛋白酶、Asp-N、Glu-C、Lys-C及Lys-N）处理，酶解为适于液相色谱-质谱联用（Liquid Chromatography-Mass Spectrometry，LC-MS）分析的肽段。本实验所用质谱仪为Q Exactive（赛默飞世尔科技，Thermo Scientific）。针对测序与肽谱分析，采用BiopharmaLynx 1.3软件对数据进行处理；针对分子量（MW）测定，则通过ProMass for Xcalibur™软件进行去卷积计算以获取相关数据。