Full-length RNA transcript sequencing traces brain isoform diversity in house mouse natural populations

Using a newly developed full-length transcripts enrichment protocol with 5’ CAP selection, we sequenced full-length RNA transcripts of 48 individuals from outbred populations and subspecies of Mus musculus, and from the closely related sister species Mus spretus and Mus spicilegus as outgroups. The dataset represents the most extensive full-length high-quality isoform catalog at the population level to date.

本研究采用新开发的、带有5’帽结构（5’ CAP）筛选的全长转录本富集方案，对来自远交种群、小家鼠（Mus musculus）亚种，以及作为外类群的近缘姊妹种——西班牙小家鼠（Mus spretus）与斯氏小家鼠（Mus spicilegus）的48个个体的全长RNA转录本开展了测序。该数据集是目前为止规模最大的种群级全长高质量转录异构体（isoform）目录。