Single-cell characterization of transcriptomic heterogeneity in lymphoblastoid cell lines

We report single-cell RNA sequencing datasets for three lymphoblastoid cell lines (LCLs) prepared by infection of primary B cells with Epstein-Barr Virus (EBV). Inter- and intra-sample transcriptional variance is observed with respect to immunoglobulin heavy chain class; host gene sets involved in survival, proliferation and differentiation; and viral replication state. We propose that the observed heterogeneity arises from the dynamic nature of host-pathogen interactions, donor-specific compositions of primary B cells, and other experimental factors such as stochastic clonal evolution in cell culture conditions. Characterization of three different lymphoblastoid cell lines. Two samples (LCL_777_B958 and LCL_777_M81) were derived from the same donor and infected with different EBV strains (B95-8 and M81). A third sample (LCL_461_B958) was derived from a different donor and infected with B95-8.

本研究报道了3株淋巴母细胞细胞系（lymphoblastoid cell lines, LCLs）的单细胞RNA测序（single-cell RNA sequencing）数据集，该细胞系通过EB病毒（Epstein-Barr Virus, EBV）感染原代B细胞构建获得。研究在免疫球蛋白重链类别、参与细胞存活、增殖与分化的宿主基因集，以及病毒复制状态这几个层面，均观测到样本间与样本内的转录组差异。我们认为，本次观测到的异质性源于宿主-病原体互作的动态特性、供体特异性原代B细胞组成，以及细胞培养环境中随机克隆进化等其他实验因素。本研究对3株不同的淋巴母细胞细胞系进行了表征：其中两个样本（LCL_777_B958与LCL_777_M81）源自同一供体，分别感染了不同的EB病毒毒株（B95-8与M81）；第三个样本（LCL_461_B958）源自另一供体，感染的EB病毒毒株为B95-8。