Bilateral recordings from dorsal hippocampal area CA1 from rats transported on a model train and sleeping.

Action potentials and local field potentials (LFPs) were recorded from the dorsal
hippocampus (CA1) of five Long-Evans rats during sleep and behavior. During behavior,
the rats were placed on a miniature treadmill transported by a model train. Passive
travel disrupts theta spike phase precession, unless the animals actively walk on a
treadmill (Cei et al., 2014). In subsequent subsessions, the treadmill was either turned
off or on to selectively perturb theta phase precession or leave it intact. Perturbation
of theta phase precession impaired theta sequences and subsequent sleep replay. This
provided evidence that theta sequences are critical to store initial memory traces that
are subsequently replayed for memory consolidation. Results from the experiments are described in:
C. Drieu, R. Todorova and M. Zugaro (2018). Nested Sequences of Hippocampal Assemblies During
Behavior Support Subsequent Sleep Replay. Science.

动作电位与局部场电位（local field potentials, LFPs）采自五只Long-Evans大鼠的背侧海马CA1区，实验覆盖睡眠与行为任务两个阶段。行为任务阶段中，大鼠被安置于由模型列车牵引的微型跑步机上。被动移动会破坏θ锋电位相位前置现象，但若动物主动在跑步机上行走则不会出现该效应（Cei等，2014）。在后续的实验亚阶段中，研究者通过开启或关闭跑步机，选择性地干扰θ相位前置现象或使其保持完好。对θ相位前置的干扰会损害θ序列以及后续的睡眠重放过程。本实验结果证实，θ序列对于存储初始记忆痕迹至关重要，而这些痕迹后续会被重放以完成记忆巩固。本实验的详细研究结果已发表于：C. Drieu、R. Todorova与M. Zugaro（2018）发表于《Science》的论文《行为期间海马集群的嵌套序列支持后续睡眠重放》。