Transcriptome analysis during seed germination of elite Chinese bread wheat cultivar Jimai 20. Triticum aestivum

Wheat seed germination is highly related to seedling survival rate and subsequent vegetative growth,and therefore directly affects the conformation of wheat yield and quality. So wheat seed germination is not only important to itself, but the whole human society. However, due to the large genome size, many studies related to wheat seed are very complex and uncompleted. Transcriptome analysis of elite Chinese bread wheat cultivar Jimai 20 may provides a comprehensive understanding of wheat seed germination. Seed germination involves in the regulation of large number of genes, whether these genes are normal activated or not is very important to seed germination. We performed microarray analysis using the Affymetrix Gene Chip to reveal the gene expression profiles in five phases of wheat cultivar Jimai 20 seed germination. Our results provide a new insights into the thoroughly metabolic changes of seed germination as well as the relationship between some significant genes. Overall design: The five groups including germinating seeds were harvest at five successive phases, which were 0 (P0), 12 (P1), 24 (P2), 36 (P3), 48 (P4) hour after imbibition respectively. Three independent experiments were performed for each group.

小麦种子萌发与幼苗成活率及后续营养生长密切相关，因此直接影响小麦产量与品质的形成。故而小麦种子萌发不仅对其自身至关重要，更与整个人类社会息息相关。然而由于小麦基因组规模庞大，诸多与小麦种子相关的研究往往极为复杂且尚未完成。对中国优质面包小麦品种济麦20（Jimai 20）进行转录组分析，或可全面解析小麦种子萌发的相关机制。种子萌发过程涉及大量基因的调控，这些基因能否正常激活对种子萌发至关重要。本研究采用Affymetrix基因芯片（Affymetrix Gene Chip）开展微阵列分析，以揭示济麦20种子萌发五个阶段的基因表达谱。本研究结果为深入解析种子萌发过程中的代谢变化以及部分关键基因间的关联提供了新的视角。总体实验设计：本次实验设置五组萌发种子样本，分别在吸胀后0（P0）、12（P1）、24（P2）、36（P3）、48（P4）小时五个连续阶段采集，每组均设置三次独立生物学重复。