DataSheet1_Claroideglomus etunicatum enhances Pteris vittata L. arsenic resistance and accumulation by mediating the rapid reduction and transport of arsenic in roots.csv

Arbuscular mycorrhizal fungi (AMF) have been widely shown to significantly promote the growth and recovery of Pteris vittata L. growth and repair under arsenic stress; however, little is known about the molecular mechanisms by which AMF mediate the efficient uptake of arsenic in this species. To understand how AMF mediate P. vittata arsenic metabolism under arsenic stress, we performed P. vittata root transcriptome analysis before and after Claroideglomus etunicatum (C. etunicatum) colonization. The results showed that after C. etunicatum colonization, P. vittata showed greater arsenic resistance and enrichment, and its dry weight and arsenic accumulation increased by 2.01–3.36 times. This response is attributed to the rapid reduction and upward translocation of arsenic. C. etunicatum enhances arsenic uptake by mediating the MIP, PHT, and NRT transporter families, while also increasing arsenic reduction (PvACR2 direct reduction and vesicular PvGSTF1 reduction). In addition, it downregulates the expression of ABC and P-type ATPase protein families, which inhibits the compartmentalization of arsenic in the roots and promotes its translocation to the leaves. This study revealed the mechanism of C. etunicatum-mediated arsenic hyperaccumulation in P. vittata, providing guidance for understanding the regulatory mechanism of P. vittata.

大量研究已证实，丛枝菌根真菌（Arbuscular mycorrhizal fungi, AMF）可显著促进砷胁迫下蜈蚣草（Pteris vittata L.）的生长与修复，但目前对AMF介导该植物高效吸收砷的分子机制仍知之甚少。为阐明AMF在砷胁迫下如何调控蜈蚣草的砷代谢过程，本研究对幼套近明球囊霉（Claroideglomus etunicatum, C. etunicatum）定殖前后的蜈蚣草根系开展了转录组分析。结果显示，经C. etunicatum定殖后，蜈蚣草的砷抗性与富集能力显著提升，其干重与砷积累量较对照组提升2.01~3.36倍，该响应归因于砷的快速还原与向上转运过程。C. etunicatum通过调控MIP（Major Intrinsic Protein）、PHT（Phosphate Transporter）及NRT（Nitrate Transporter）转运蛋白家族的表达增强砷吸收，同时促进砷还原过程，包括PvACR2介导的直接还原与囊泡内PvGSTF1（谷胱甘肽S-转移酶F1）介导的还原。此外，该真菌可下调ABC（ATP-binding cassette）与P型ATP酶（P-type ATPase）蛋白家族的表达，从而抑制砷在根系中的区室化，促进其向叶片转运。本研究揭示了C. etunicatum介导蜈蚣草砷超富集的分子机制，为解析蜈蚣草的砷调控通路提供了理论指导。