VEGFR3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection. VEGFR3 signaling restrains the neuron-macrophage crosstalk during neurotropic viral infection

To investigate the transcriptomic characteristics of CD11b+F4/80+ cells in the brain of JEV- infected WT and Vegfr-3ΔLBD/ΔLBD mice, we sorted the CD11b+F4/80+ cells from the brain of WT and Vegfr-3ΔLBD/ΔLBD mice post 5 days of JEV infection by FACS. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CD11b+F4/80+ cells in the brain of JEV- infected WT and Vegfr-3ΔLBD/ΔLBD mice.

为探究日本脑炎病毒（Japanese Encephalitis Virus, JEV）感染后野生型（Wild Type, WT）与血管内皮生长因子受体3（Vascular Endothelial Growth Factor Receptor 3, Vegfr-3）配体结合域缺失纯合突变（Vegfr-3ΔLBD/ΔLBD）小鼠脑内CD11b+F4/80+细胞的转录组特征，本研究于病毒感染5天后，通过流式细胞分选（Fluorescence-Activated Cell Sorting, FACS）从两类小鼠的脑组织中分离得到CD11b+F4/80+细胞。随后基于两组不同来源细胞的RNA测序（RNA-sequencing, RNA-seq）数据开展基因表达谱分析。
整体实验设计：对JEV感染的WT与Vegfr-3ΔLBD/ΔLBD小鼠脑内CD11b+F4/80+细胞的RNA测序数据进行对比基因表达谱分析。