Genes related to microtubule functions were downregulated in sinhcaf mutant FG follicles and eggs

Transcriptome sequencing (RNA-seq) analysis uncovered that 1238 genes were upregulated, 923 genes were downregulated in sinhcaf mutant FG follicles, compared to wild type FG follicles. Loss of sinhcaf in mature eggs resulted in upregulation of 904 genes, and downregulation of 911 genes. Downregulated genes in sinhcaf mutant FG follicles and eggs were classified by gene ontology (GO) analysis, then the top 30 GO terms with highest -log10P values were screened out, and at least two downregulated genes were included in each biological processes, cellular components or molecular function term. Microtubules and microtubule-based processes were included in the top 30 GO terms. Overall design: RNA sequencing was performed on five biological replicates for mature egg, four biological replicates for FG follicle of wild type and sinhcaf mutant zebrafish. Each biological replicate was a pool of mature eggs or FG follicles from one female zebrafish.

转录组测序（RNA-seq）分析结果显示，相较于野生型FG卵泡，sinhcaf突变型FG卵泡中共有1238个基因上调、923个基因下调。成熟卵子中sinhcaf功能缺失会导致904个基因上调、911个基因下调。针对sinhcaf突变型FG卵泡与卵子中的下调基因开展基因本体（GO）分析并进行分类，随后筛选出-log₁₀P值最高的前30个GO条目，且每个生物过程、细胞组分或分子功能条目均至少包含2个下调基因。微管及基于微管的生物过程被纳入该前30个GO条目之中。实验设计：针对野生型与sinhcaf突变型斑马鱼，对其成熟卵子设置5个生物学重复样本，对其FG卵泡设置4个生物学重复样本并开展RNA测序；每个生物学重复样本均取自单条雌性斑马鱼的成熟卵子或FG卵泡的混合样本。