Leptospira MinION direct cDNA sequencing

Transcriptome sequencing for 4 different Leptospira serovars, both pathogenic (L. interrogans: Copenhageni; Icterohaemorrhagiae; Mankarso) and non-pathogenic (L. biflexa: Patoc) using ONT's direct cDNA sequencing with and without enzymatic polyadenylation本数据集针对4种不同的钩端螺旋体（Leptospira）血清型开展转录组测序（Transcriptome sequencing），涵盖致病型与非致病型菌株：致病型菌株为问号钩端螺旋体（L. interrogans）的Copenhageni、Icterohaemorrhagiae及Mankarso血清型，非致病型菌株为双曲钩端螺旋体（L. biflexa）的Patoc血清型；测序采用牛津纳米孔技术（ONT，Oxford Nanopore Technologies）的直接cDNA测序方案，分别设置带有酶促多腺苷酸化（enzymatic polyadenylation）与不带有酶促多腺苷酸化的两组实验条件。