o Project 60 (Version 7.0): Spatial Transcriptomics, Microenvironmental Partitioning, and Kinetic Simulation Framework for Pancreatic Ductal Adenocarcinoma (PDAC)

Comprehensive research framework and repository for Project 60 (Version 7.0). This dataset and code bundle integrates single-cell and spatial transcriptomics from human Pancreatic Ductal Adenocarcinoma (PDAC) biopsies (NCBI GEO: GSE274103) with a non-linear Michaelis-Menten kinetic model and multi-layered molecular biocomputing. It features spatial domain partitioning via Scanpy (highlighting Cluster 7 as the invasive/proteolytic niche with enriched MMP9 and PI15), a two-wave nanotherapeutic delivery architecture designed to overcome desmoplastic stroma and therapeutic resistance, and full simulation matrices for conditional cargo release.