Gene expression profile in the tethered Nrf1-MafG (T-N1G) heterodimer-expressing small Maf-deficient cells [RNA-seq]. Gene expression profile in the tethered Nrf1-MafG (T-N1G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

To elucidate the function of Nrf1-small Maf heterodimer, we constructed a tethered Nrf1-MafG (T-N1G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF). Overall design: polyA RNA-seq was performed using RNA from immortalized MafF-/-::MafG-/-::MafK-/- (F0G0K0) MEFs and T-N1G-expressing F0G0K0 MEFs, treated with Vehcle, 0.3 µM or 1.0 µM Proteasome Inhibitor (MG132) for 12hr.

为阐明Nrf1-小Maf异二聚体（Nrf1-small Maf heterodimer）的功能，我们通过柔性连接肽（flexible linker peptide）构建了拴系型Nrf1-MafG（T-N1G）异二聚体，并在小Maf缺陷型小鼠胚胎成纤维细胞（MEF，mouse embryonic fibroblast）中对其功能进行了检测。实验总体设计如下：分别以永生化MafF^-/-::MafG^-/-::MafK^-/-（F0G0K0）MEFs及表达T-N1G的F0G0K0 MEFs的RNA开展polyA富集RNA测序（polyA RNA-seq）；所有受试细胞均经溶剂、0.3 μM或1.0 μM蛋白酶体抑制剂（MG132）处理12小时。