Methyl-CpG-binding domain (MBD) sequencing of 45 primary neuroblastoma tumors

The DNA methylome of 45 primary neuroblastoma tumors is profiled by enrichment with a methyl-CpG-binding domain (MBD) and massively parallel sequencing Overall design: DNA of 45 primary tumors is sheared (fragments of ± 200 bp), followed by MBD-based (MethylCap kit of Diagenode) enrichement, library preparation and multiplexing. Both input DNA and captured DNA were sequenced paired-end on Illumina Hiseq2000

本数据集通过甲基-CpG结合结构域（methyl-CpG-binding domain, MBD）富集结合大规模平行测序（massively parallel sequencing）技术，对45例原发性神经母细胞瘤（neuroblastoma）肿瘤的DNA甲基化组进行了表征分析。
实验整体设计如下：首先将45例原发性肿瘤的DNA进行片段化处理，得到长度约±200 bp的DNA片段；随后使用Diagenode公司的MethylCap试剂盒开展基于MBD的富集实验，继而完成文库制备与多重标记（multiplexing）。输入DNA与捕获得到的DNA均在Illumina Hiseq2000测序平台上进行双端（paired-end）测序。