Effect of adipose tissue insulin resistance on gene expression of liver in diet-induced NASH model.

To investigate the alterlation of liver gene expression in diet-induced NASH model with adipose tissue insulin resistance, we evaluated liver of control mice and adipocyte-specific PDK1 knockout (PDK1KO) mice fed either normal chow(NC) or Gubra amylin NASH (GAN) diet for 16weeks after weaning at 4 weeks old. We then performed gene expression profiling analysis using data obtained from RNA-seq of these 4 groups. Overall design: Comparative gene expression profiling analysis of RNA-seq data for liver of 4 groups[Control(NC), Contro(GAN), PDK1KO(NC),PDK1KO(GAN)].

为探究合并脂肪组织胰岛素抵抗的饮食诱导非酒精性脂肪性肝炎（NASH）模型中肝脏基因表达的改变，我们以4周龄断奶的小鼠为实验对象，分别饲喂正常饲料（normal chow, NC）或Gubra胰淀素NASH（GAN）饲料，持续造模16周后，采集对照小鼠与脂肪细胞特异性丙酮酸脱氢酶激酶1敲除（PDK1KO）小鼠的肝脏组织。随后基于上述4组样本的RNA测序（RNA-seq）数据开展基因表达谱分析。整体实验设计：对4组小鼠肝脏的RNA测序数据进行对比基因表达谱分析，4组分别为对照+正常饲料组（Control(NC)）、对照+GAN饲料组（Control(GAN)）、PDK1KO+正常饲料组（PDK1KO(NC)）以及PDK1KO+GAN饲料组（PDK1KO(GAN)）。