Comparing gene-expression profiles between control and CHD6 knock-down HCT116 cells

Colorectal cancer is one of the most common cancers. Cancer cells are highly dependent on dysregulated gene expression to support their uncontrolled growth and high energy needs. The expression of gene encoding chromodomain helicase DNA binding protein 6 (CHD6) is frequently altered in tumor tissues of colorectal cancer patients.We performed transcriptomic analysis to identify the differentially expressed genes between control and CHD6 KD HCT116. Colorectal cancer cell line HCT116 with Tet-on inducible CHD6 KD construct was treated with doxycycline or vehicle for 48h. And cells were collected for RNA extraction. The RNA samples were hybridized on Affymetrix microarrays.

结直肠癌是最常见的恶性肿瘤之一。癌细胞高度依赖基因表达失调，以维持其不受控制的增殖与旺盛的能量需求。编码染色质域解旋酶DNA结合蛋白6（chromodomain helicase DNA binding protein 6，CHD6）的基因在结直肠癌患者的肿瘤组织中常出现表达异常。我们通过转录组学分析，筛选了对照组与CHD6敲低（CHD6 KD）HCT116细胞间的差异表达基因。将携带四环素诱导型CHD6敲低载体的结直肠癌细胞系HCT116以多西环素或溶剂对照处理48小时后，收集细胞进行RNA提取。将提取的RNA样本在Affymetrix基因芯片上进行杂交。