Transcript analysis under calcium deficiency in 6 day old Marchantia polymorpha wildtype (Tak-1) and Gβ-null mutant (gpb1-2) gemmalings and inter-species comparison of M. polymorpha, Arabidopsis and lettuce under calcium deficiency

Calcium deficiency response in liverwort, Arabidopsis and lettuce: (1) Marchantia polymorpha: M. polymorpha wildtype and Gβ-null mutant plants (Tak-1, gpb1-2) were grown in control liquid Yamagami media (2 mM Ca) for 6 days. For RNA-seq experiments, 6 day old gemmalings were transferred to calcium deficiency (0 mM Ca) media. Samples were collected at 48 h after the transfer. The transcriptomic profiles were collected from two independent batches. In total four biological replicates were used for each condition and each genotype for a total of 16 samples. (2) Arabidopsis thaliana: For Arabidopsis RNA-seq experiment, 6-day old seedlings grown on ½ strength MS media with sucrose were transferred to Yamagami media with 2 mM or 0 mM CaCl2 and treated for 7 days. (3) Lactuca Sativa: For lettuce RNA-seq, 4-day old seedlings grown on water agar (1%) were transferred to Yamagami media with 2 mM or 0.15 mM CaCl2 and treated for 7 days. In total four and three biological replicates were used for each condition for a total of 8 and 6 samples respectively for Arabidopsis and lettuce. Transcript analysis of 6-day old M. polymorpha gemmalings under two conditions (2 mM Ca, 0 mM Ca) with wild type Tak-1 and gpb1-2. Transcript analysis of 6-day old (Arabidopsis) under two conditions (2 mM Ca, 0 mM Ca) and 4-day old (lettuce) wildtype seedlings under two conditions (2 mM Ca, 0.15 mM Ca).

地钱（Marchantia polymorpha）、拟南芥（Arabidopsis thaliana）与生菜（Lactuca sativa）的钙缺乏响应数据集：
(1) 地钱：将其野生型与Gβ缺失突变体植株（Tak-1、gpb1-2）在对照液体山上文（Yamagami）培养基（含钙2 mM）中培养6天。针对RNA测序（RNA-seq）实验，将6日龄芽孢幼苗（gemmalings）转移至钙缺乏（含钙0 mM）培养基中，于转接后48小时采集样本。转录组数据来自两个独立批次，每个培养条件与每个基因型均设置4个生物学重复，总计16个样本。
(2) 拟南芥：将在含蔗糖的1/2强度MS培养基上培养6天的幼苗，转移至含钙2 mM或0 mM CaCl₂的山上文培养基中，处理7天。
(3) 生菜：将在1%水琼脂培养基上培养4天的幼苗，转移至含钙2 mM或0.15 mM CaCl₂的山上文培养基中，处理7天。其中拟南芥每个培养条件设置4个生物学重复，总计8个样本；生菜每个培养条件设置3个生物学重复，总计6个样本。
本数据集的转录组分析涵盖：① 两种钙浓度条件（2 mM Ca、0 mM Ca）下、携带两种基因型（野生型Tak-1与gpb1-2）的6日龄地钱芽孢幼苗；② 两种钙浓度条件（2 mM Ca、0 mM Ca）下的6日龄拟南芥幼苗，以及两种钙浓度条件（2 mM Ca、0.15 mM Ca）下的4日龄生菜野生型幼苗。