Hypothalamus of lean mice treated with celastrol or DMSO. Hypothalamus of lean mice treated with celastrol or DMSO

Given that celastrol?s leptin-sensitizing effect requires high levels of circulating leptin, but lean mice have low levels of circulating leptin so that celastrol has no effect on lean mice. Analysis celastrol-induced hypothalamic gene expression profile change in lean mice will also be serving as negative control for DIO mice analysis. Overall design: lean mice were injected with celastrol or DMSO as vehicle control for 6h, 1day or 4 days and then the RNA was extracted from hypothalamus for microarray

鉴于雷公藤红素（celastrol）的瘦素增敏作用需要高水平循环瘦素（leptin），而瘦型小鼠的循环瘦素水平较低，因此雷公藤红素对瘦型小鼠无作用。对瘦型小鼠体内雷公藤红素诱导的下丘脑基因表达谱变化进行分析，亦可作为饮食诱导肥胖（DIO）小鼠相关分析的阴性对照。实验设计：将瘦型小鼠分别注射雷公藤红素或作为溶剂对照的二甲基亚砜（DMSO），分别于处理6小时、1天或4天后，从其下丘脑中提取RNA，用于基因芯片（microarray）检测。