miRNA data in human placentas from normal pregnancies and from pregnancies complicated by SGA and IUGR. Homo sapiens

Placental insufficiency leading to intrauterine growth restriction demonstrates perturbed gene expression affecting placental angiogenesis and nutrient transfer from mother to fetus. To understand the post-transcriptional mechanisms underlying such placental gene expression changes, our objective was to identify key non-coding microRNAs that express biological function. To this end, we undertook microarray targeting microRNAs in placentas of appropriate (AGA, n=3) versus small for gestational age (SGA, n=3) weight infants and observed upregulation of 97 miRs in SGA versus AGA. In a larger cohort of samples, we validated by qRT-PCR, differential expression of three specific microRNAs that target genes mediating angiogenesis and nutrient transfer. We performed microarray on a small cohort of samples in order to identify differential expression in pathways involved with angiogenesis and nutrient transfer, essential processes for placental function that when perturbed, may result in intrauterine growth restriction. Overall design: Human placental samples were obtained immediately post-parturient. There were three samples from the AGA group (Bw>10% and <90%) and three samples from the SGA/IUGR (Birthweight <10%) group. All placental samples were from term, singleton pregnancies.

胎盘功能不全（placental insufficiency）引发的宫内生长受限（intrauterine growth restriction, IUGR）会导致基因表达紊乱，影响胎盘血管生成（angiogenesis）以及母体向胎儿的营养转运（nutrient transfer）过程。为阐明此类胎盘基因表达改变背后的转录后调控机制，本研究旨在鉴定具有生物学功能的关键非编码微小RNA（microRNA, miRNA）。

为此，我们针对适于胎龄儿（appropriate for gestational age, AGA，n=3）与小于胎龄儿（small for gestational age, SGA，n=3）的胎盘组织开展了靶向微小RNA的基因芯片（microarray）检测，结果发现SGA组相较于AGA组有97种微小RNA表达上调。在更大规模的样本队列中，我们通过实时定量逆转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction, qRT-PCR）验证了3种靶向调控血管生成与营养转运相关基因的特定微小RNA的差异表达情况。

我们针对小样本队列开展基因芯片检测，旨在筛选参与血管生成与营养转运通路的差异表达微小RNA——这两类过程是胎盘功能的核心环节，一旦出现紊乱即可引发宫内生长受限。

实验设计：所有人类胎盘样本均于产后即刻采集。AGA组（出生体重处于10%~90%百分位区间）共3例样本，SGA/IUGR组（出生体重低于10%百分位）共3例样本。所有胎盘样本均来自足月单胎妊娠。