Compounds from the US drug library screened with EcaGST A3-3 giving 30% to 50% inhibition.

Enzymatic activity was measured with 1 mM GSH and 1mM CDNB with and without 10 μM inhibitor in sodium phosphate buffer at pH 6.5 and 30°C.本实验于pH 6.5、30℃的磷酸钠缓冲液中开展，反应体系包含1 mM谷胱甘肽（GSH）与1 mM 1-氯-2,4-二硝基苯（CDNB），分别设置添加10 μM抑制剂与不添加抑制剂两组，以此测定酶活性。