MOESM1 of Cell-traversal protein for ookinetes and sporozoites (CelTOS) formulated with potent TLR adjuvants induces high-affinity antibodies that inhibit Plasmodium falciparum infection in Anopheles stephensi

Additional file 1: Figure S1. Western blot analysis of expressed PfCelTOS in E. coli. Figure S2. Indirect immunofluorescence antibody test (IFAT). Figure S3. Comparison of the anti-rPfCelTOS IgG, IgG1, IgG2a, IgG2b, and IgG3 antibodies in all vaccinated mice groups (1â 5) with the control mice groups (6â 10) on day 38 of the first immunization. Figure S4. Lymphocyte proliferation in cultured splenocyte cells of immunized mice (groups 1â 10) in the presence of rPfCelTOS in vitro.

附加文件1：图S1。大肠杆菌（E. coli）中表达的PfCelTOS的蛋白质免疫印迹（Western blot）分析。图S2：间接免疫荧光抗体试验（Indirect immunofluorescence antibody test, IFAT）。图S3：首次免疫后第38天，所有免疫小鼠组（1~5组）与对照小鼠组（6~10组）的抗重组PfCelTOS（rPfCelTOS）IgG、IgG1、IgG2a、IgG2b及IgG3抗体水平比较。图S4：体外添加重组PfCelTOS（rPfCelTOS）时，免疫小鼠（1~10组）的培养脾淋巴细胞增殖情况。